Fig. 4. Cell viability and cell death induced by tBHP or ROT in WT and VDAC1^-/- H9c2 cells. (A) MTT assay (cell viability) of WT and VDAC1^-/- C1, C2 and C3 H9c2 cells after 20 h exposure with various concentrations of tBHP. (B) TUNEL assay of WT and VDAC1^-/-C1 H9c2 cells after 20 h exposure to various concentrations of tBHP. (C) Representative TUNEL staining (cell death) of WT and VDAC1^-/-C1 H9c2 cells after 20 h exposure to 125 μM of tBHP. Apoptotic nuclei were TUNEL stained (red) and counterstained with DAPI (blue) to label nuclei. The red and blue merged cells were counted as TUNEL positive cells. (D) LDH release from WT, VDAC1^-/-C1, C2 and C3 and VDAC1 restored in VDAC1^-/-C1 H9c2 cells after 3 h exposure to 50, 100 μM tBHP. (E) MTT assay of WT and VDAC1^-/-C1, C2 and C3 H9c2 cells viability after 48 h exposure to various concentrations of ROT. *P<0.05 vs WT, ^# P<0.05 vs VDAC1^-/-. Each experiment was repeated three times.